![]() ![]() There is an increase the likelihood for finding enzymes with distinctive characters in area ranging from dry deserts to extreme cold Antarctic regions. In industrial sector enzymes have immense applications, among these enzymes microbial ones form an inevitable part and have always been in demand due to their unique properties (Liu and Kokare 2017). Enzymes can be isolated from any living forms and each one has its own characteristic property which separates it from other. The importance of microbial enzyme lies in the role played by these bio-molecules in catalyzing the broad array of reactions which is indispensable for maintaining life in earth. Available from: Copyright © Vivek et al., This is an Open Access Article distributed under the Terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use distribution and reproduction in any medium, provide the original author and source are credited. Screening, Production and Characterization of Industrially Important Enzymes by Serratia marcescens Strain VT 1. The wide range pH active protease and psychrophilic and organic solvent stable lipase can be made use in various sectors like bioremediation, waste management, and chemical synthesis, food processing and detergent additives. Lipase showed stability in both hydrophobic and hydrophilic solvents, hexane- 92.67%, methanol-87.4% and acetone-85.23%. SMVT 1 protease was stable in hydrophilic solvents like methanol and ethanol. SMVT1 lipase was found to be active from pH 7-9 with optimum pH 7 and good stability for 90 minutes, optimum temperature 30☌ and to retain almost 80 % activity at lower temperatures (20 and 10☌). Characterization of protease showed the enzyme to be active over a wide range of pH 4-11, optimum pH 10, and optimum temperature 50☌. A mesophilic novel strain of Serratia marcescens – VT1 was found to produce extracellular protease (13.173 U/ml) and cold active and stable psychrophilic lipase (23.17 U/ml). Microorganisms and their enzymes are important part of industrial biotechnology because of their high adaptability, versatile metabolic machinery and simple genetic constitution which can be easily manipulated to meet different needs. ![]()
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